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However, although control cells could sustain their viability in cysteine-depleted medium, the CBS -deleted cells did not (Figure 1E), which is in agreement with a greater depletion of cellular GSH levels in CBS -deleted cells compared to control cells following cysteine deprivation (Figure S1I)

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Thus, activation of NFκB by ROS (or other signaling pathways) upregulates the expression and activity of ROS generators, such as NADPH oxidase 1 and 2 (NOX1 and NOX2) that produce superoxide [9, 10]