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A total of 5 μg His-UBE2m and 5 μg GST-PRMT1 were added into a standard 50-μL reaction mixture containing 5 μl S-adenosyl-l-methionine (SAM, Amersham Bioscience), 50 mM Tris-HCl (pH 8.0), 20 mM KCl, 5 mM DTT, and 4 mM EDTA

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